Researcher Joshua Leisk, a former CFS/ME sufferer and IT professional with a keen interest in bioinformatics, has been symptom-free for 25 years. He began researching CFS/ME out of curiosity, focusing on liver and brain interactions. Through analyzing health data from diet coaching clients, he identified common patterns in urea cycle abnormalities and adrenal cortex dysregulation.
His research suggests that latent viral infections like EBV significantly contribute to CFS/ME. He found that personalized diet and supplement interventions improved his clients' symptoms. Leisk, despite lacking formal academic credentials, collaborated with his partner, a Neuroimmunology PhD, to publish papers on his findings.
Leisk's goal is to help others without financial gain. He has filed a patent for his treatment method and seeks assistance in advancing his research and conducting clinical trials. His work is now expanding to explore connections with other disorders and diseases.
Here is his story, in his own words...
The Origin of This Discovery
Editor's note: This backstory was first shared at S4ME and then Phoenix Rising, in early 2021 and has minor edits for clarity.
I'm an anomaly.
I'm a fully recovered CFS/ME sufferer (25 years free and clear) with a strong background in IT and and interest in bioinformatics. I "retired" at 38 and got bored. I like puzzles, pathways and systems. This was a puzzle that had bothered me for a few decades, so I thought it was worth helping people solve.
Here are our three most recent papers, currently in preprint:
https://www.researchgate.net/publication/350043016_The_true_nature_of_an_autoimmune_disease
https://www.researchgate.net/publication/350399073_The_involvement_of_acetate_aspartate_butyrate_and_enzymatic_cofactors_in_the_HASD_CFSME_model
https://www.researchgate.net/publication/350956432_CFSME_A_New_Hope
For CFS/ME, our primary research focus is the liver (and now also the brain).
The Original Research
My original research strongly suggested that it's a combination of the antibodies from the lytic phase, PLUS the primary issue of latent viral hepatitis - the metabolic burden of the latent cell activities, hard-wired by GLS1 - KGA, GAC + GDH, etc., for protein-synthesis tasks, glutaminolysis and lactate production interfere by the behaviour of the neighbouring hepatic cells. eg Lactate cycle metabolism. Arresting the lytic phase does not solve CFS/ME, but it will reduce the antibodies.
Checking eg. EBV EBNA,VCA IgG counts for systemic latent infection and then inferring localised hepatic infection by performing the 'succinate challenge' I mentioned in the paper gives us an understanding of the pathophysiology, where hypoxia is present. For other disorders and diseases, it's largely tissue specific.
We would happily perform more experiments ourselves, but we're a pair of researchers without a lab facility.
We would love to run a clinical trial based on some robust preliminary test results, however we are reliant on others for properly testing this hypothesis. We are currently engaging with some existing parties to make that happen here in Australia, although are open to assistance from any other parties.
We're happy to work with others. In case we have misinterpreted any cited papers, we're open to any discussion and correction.
Clients Lead to More Discoveries
The background on this discovery is that in the course of my normal consulting, I had a cluster of 5 diet coaching clients over roughly one month presenting for a number of different goals, yet similar issues - with a common complaint of daytime fatigue.
Most had joint pains and involuntary muscle contractions. The male and female clients had endocrine, sleeping and neurological disorders, such as acute anxiety. The males and females exhibited signs of alopecia. All had extensive pathology data, spanning more than 10 years and a history of symptoms longer than 15 years. Only one client had ever been officially labeled as a "CFS/ME" patient.
I compiled and analyzed their pathology data and saw a common pattern of urea cycle abnormalities, cortisol / adrenal cortex dysregulation and mild leukopenia - specifically, borderline subclinical lymphocytes. ANA, CK, cRP, ESR, thyroid markers all unremarkable. Some minor liver enzyme elevations in some clients, which appeared to match their body composition and life choices. I thought the overall pattern was interesting, so I kept investigating.
Most clients had acute eating disorders. Ear/nose/throat infections were common. Environmental allergies were common. There was a history of GI issues and all had a specific pattern of food intolerances. Eggs and lecithin were consistently mentioned. I found that even more interesting.
I assessed their dietary habit using one of my favourite nutrition tracking tools, "Cronometer". The observed protein intake was very low - typically less than 40 grams per day, which did not match the unusual serum urea also being observed - BUN was typically high range, or in one client, very low range. This was interesting, because it suggested that either glutaminolysis was being used, and / or the urea cycle was impaired at different times.
Three of the clients had reported some benefits from ketogenic diets. One of them was currently employing a ketogenic diet. The others had not attempted this. This one client chose to discontinue their ketogenic diet and had an acute worsening of symptoms. This was interesting because it demonstrated a pattern of mitochondrial impairments.
All clients had a habitual lifestyle that obsessively revolved around dietary supplements. This was also interesting and it allowed me to ask them what supplements they took regularly and why.
Importantly, I also asked them what they didn't take and why: "What had they experienced negative reactions to?"
A common list of "problem" supplements for all clients was acetyl-l-carnitine, EGCG, choline, arginine, citrulline. Some of them reported that acetyl-l-carnitine caused acute edema and myopathy. I found that very interesting, also - suggesting influences to fatty acid oxidation / PDH, GDH, acetylcholine receptors, respectively.
All of the clients had exercise intolerance to even the mildest exertion levels, with suboptimal lactate threshold and oxygenation, even beyond my expectations for a sedentary lifestyle.
Three of the clients had prescriptions for salbutamol inhalers.
From this combined data, I saw more patterns forming. I analyzed other data they had captured, including HTMA tests, which excluded heavy metals, etc as a source of hypoxia.
My Work to Help Progresses
At this point, I decided to take a personal interest in these cases and chose to work closely with two of these clients on a daily basis, exploring published literature and talking with them for typically five-eight hours a day over many months, observing and analysing high levels of detail about their diet, daily activities, symptoms, influences / triggers / responses, while assembling connections between individual data points. This was a performed without any financial consideration. Being "retired" made this level of dedication and focus possible.
Dietary intervention of adding two eggs and some soy lecithin to every meal and 2 more before bed improved or resolved involuntary muscle spasms, with some not-unexpected discomforts. This was partially replaced by choline bitartate.
Pathology for Plasma Amino Acids found some common abnormalities with elevated glutamine, glutamic acid, low 1-methyl-histidine.
Exploring the literature and looking at the published CFS/ME metabolomics data, I saw many parallels between client data and published data. I explored this further, creating new connections along the way.
Serology markers showed high titre active EBV in both cases. There were differences in testing method availability, due to geographical location.
(Shared with full consent / expressed permissions.)
Client 1
EBV EBNA IgG++
EBV VCA IgG+++ (exceeded reporting thresholds)
EBV VCA IgM-
EBV EA was not provided
CMV IgG-
CMV IgM-
HSV-1++
HHV-6+
aPL-
AChR-MUSK-
Client 2
EBV EBNA was not provided
EBV VCA IgG++
EBV VCA IgM+
EBV EA was not provided
CMV IgG ++
CMV IgM -
aPL-
AChR-MUSK-
At this point, I considered that we had maybe progressed beyond "smoke" to "fire".
Questions and Answers: Going Beyond "Smoke" to "Fire"
Perhaps the impact from lytic EBV infection or CMV was the source of the symptoms?
I reached out to those two clients' physicians and requested assistance. Under their care, off-label spironolactone (aldactone) was initiated at 25mg/day (Campo et al.) and Client 1 increased to 2 x 25mg/day after one month. Client 2 also chose to self-administer nigella sativa for their CMV infection.
Over three months of close interactions, I observed many patterns in triggers / responses and durations, including associations between both clients' reported salbutamol usage vs timing of and cessation of upper-right abdominal pain, with increased energy levels.
The abdominal pain followed periods where they had been feeling better, with improved energy levels and decided to make use of them. Due to concomitant hypoxic symptoms, they self-administered salbutamol and this demonstrated a pattern of resolving their abdominal pain and also a reduction of the duration and intensity of post-exercise malaise. It also increased their energy levels. I found this very interesting.
The location of the pain and the relationship to the salbutamol dosage suggested strongly that beta-oxidation pathways were involved and the lactic acid cycle was impaired at hepatic gluconeogenesis. It didn't explain the cause of the high lactate, which I considered to be related to the impairments surrounding energy production.
Via close observation of client myopathy vs activity, I could see that unusual amounts of lactate were being generated, in addition to impairments in hepatic conversion back to glucose.
Combined with the noted urea cycle abnormalities and hypoxia, this was pointing strongly to a deficit in succinate and fumarate. The general lack of energy was suggesting an insufficient amount of ATP was being generated. This pointed to Complex V.
Gradually, over these months, I collected enough information from observations and literature searches to collectively create a hypothetical map.
Client 1 obtained a high result for EBV PCR roughly one month after starting spironolactone and flow cytometry, from the same sample showed low CD4. At two months, returned negative on PCR tests. WBC / flow cytometry all amazingly unremarkable.
Client 2 obtained negative results on both EBV and PCR test at roughly six weeks.
This was a strong suggestion that spironolactone has efficacy for arresting EBV, CMV lytic phase. Only expected side-effect being increased urination, requiring increased hydration and electrolytes.
Both clients had some tangible improvements to many symptoms, but were still showing all of the hallmark CFS/ME pattern of impairments I had personally experienced all those years ago.
The lytic phase is only partially responsible for CFS/ME. (n=2). Wonderful, so what does this mean?
Looking at the pathway map I had created, three "hot-spots" were a-KGDH, PDH and selective beta-oxidation pathway insensitivity.
NutraEval reports are incredibly useful for identifying these abnormalities, however these need to be interpreted based on the level of activity prior to sampling, in much the same way that metabolomic studies need to be controlled against time of day and prior activity levels to provide any meaningful data.
Further to this, my research into the behaviour of HHV-infected cells revealed a metabolic preference for glutaminolysis (Krishna G et al), just like many cancer cells (Song Z et al.) and an ability to replicate via transcytosis (Hutt-Fletcher L et al.). This suggested a number of things, not the least of which was that these infected cells would be susceptible to the same metabolic influences as those cancer cells (Saunier E et al.). I considered that just like in certain types of cancer cells, the location and behaviour of the latent cells would have influence on the neighbouring cells. Where these are hepatic cells, this would unduly influence the hepatic function, in particular with regards to lactate metabolism / gluconeogenesis. In other tissues, many other disorders would be expected, where collagen synthesis and other tasks downstream of prolyl hydroxylase activities are degraded, leading to various states of inflammation and cortisol dysregulation. This has implications for Ehlers Danlos Syndrome and others.
As such, in my capacity as a diet and health coach, I educated Client 1 and Client 2 on the benefits of specific over-the-counter dietary supplements which are known for addressing these pathways, with advice to verify with their physician if contraindicated in their illnesses.
In the general population, like most dietary supplements in general, these specific dietary supplements would have little to no noticeable effects or benefits beyond those provided by a normal, balanced diet. They chose to purchase these from their local health food store or supplement vendor and self-administer them. They further chose to provide me with reports on their experiences with this self-experimentation.
Unlike the typical 'non-response' expected in the general population, the effects from these specific supplements were reported as both rapid and acute in both clients. Their energy levels returned to normal and they regained normal daily functionality and lifestyles. Due to their natural curiosity and a long history of experimentation with supplements, they also tried different combinations and dose schedules of these supplements and reported the effects. I analysed their reports and noticed a pattern, where failure to address any one of these "hot-spots" I had educated them on led to a consistently repeatable pattern of initial impairments and a resumption of full CFS/ME symptomology.
Using the combination of dietary supplments, viral EBNA IgG has been demonstrated to have decreased by 25% over two months, suggesting reduced systemic burden.
We are long past "smoke," well past "fire," and currently discussing the merits of different coloured fire extinguishers.
A Grander Mission Unfolds This needs robust testing; How do I share this information with people who are in a better position to make use of it and without creating problems associated with communicating this around a demographic of patients who are desperate for early answers?
At this point, although having read perhaps 900 papers, I thought it would probably be best if I studied the literature further, wrote a review and shared it with the community. This presented some new difficulties, as although demonstrated by my recent manuscript, through personal interests my understanding of metabolism, rheumatology, cellular biology, immunology, endocrinology and biological pathways could be considered with some equivalencies to a PhD level education, due to my life choices and preference for self-education, usually by intense reading, I lack the credentials required to present these findings to a wider audience, in the format they would normally be inclined to appreciate and give due consideration.
I prefer to learn things in my own way and explore topics in an organic way, where my brain absorbs them efficiently. I find structured education traditionally 'grinds my gears' by causing frustrations and inefficiencies, therefore I limit any formal education and certifications to the barest minimum required to permit me to work in any specific field. This is also likely why I left school at 14 and "retired" at 38. Overall, I have lived an unusual life.
A self-taught "diet and health coach" traditionally does not write medical journal articles on complex metabolic disorders and virology. This is a significant anomaly, although my life to-date has been one long anomaly. Importantly, I also lacked a full understanding of the nuances and expectations of academia, with regards to publishing material for peer-review.
Fortunately, through fate and/or luck, my life-partner and co-author happens to be a brilliant scientist, holding a PhD in Neuroimmunology, with a Masters in Biochemistry. Keeping in line with my usual preference for organic learning, she helped me understand the normal requirements for publishing a paper, assisted and answered questions about lab methodology, where I found criticisms of papers I was reading, edited and helped proof the manuscript, along with many other key aspects of the journey towards where we are today. I'm always thankful for having her in my life.
In the process of continuing to map the pathophysiology, draw the diagrams and write the paper, my research connected the dots with a broader array of disorders and diseases. I realised that my research had significantly greater implications for many diseases and disorders. During the many weeks that was required to author the paper, I was was also contacted by some other clients and friends who had a number of different diseases / disorders - including bipolar disorder, schizophrenia, ehlers danlos syndrome, IBS, lupus and rheumatoid arthritis, which were already strongly hinted at having a common origin, by the growing manuscript and diagrams.
Consequently, I found the same signature of metabolic alterations and serology markers in those clients and they show an acute response to the same dietary supplement advice, although there are variations and further optimisations possible where hepatic impairment cannot be demonstrated or where lytic phase cannot be detected in serology. For each of these disease model sub-types, I have drafted early specifications that can be used for testing in clinical trial research around these disorders:
V1: There were two small mitochondrial "leaks" to resolve regarding a-KG accumulation and ROS. They're now fixed in v2 and provide a treatment which allows normal daily life, however this can be further improved.
V2 was demonstrated in small numbers to provide symptomatic remission, with gradual improvements to latent cell burden, if combined with a prescription for eg. spironolactone.
V3 is being actively tested.
Moving Forward With the Goal To Help More People
At this point:
We have also filed a patent for the formula, to also aid later discussions with pharmaceutical companies.
We are not selling any products; We are not selling any services.
The treatment involves items that can be bought at vitamin stores and supermarkets.
If people need help, I've been offering my time and assistance without any thought of financial gain. This is real and my aim is to help people.
However, this is also where WE need help in continuing this journey.
Can you, the reader, please assist us in making this a success for everyone?
Interested in Exploring the Born Free Protocol?
Discover a supportive approach to managing chronic illness with the Born Free Protocol. Our comprehensive guide is designed to provide you with insights and strategies to navigate your health journey more effectively. Whether you're looking for ways to understand your condition better or seeking new perspectives on wellness, our PDF is a valuable resource.
Download your copy today and/or join the Born Free protocol support group online at Discord and take a step towards informed health management. You can also watch a video where Leisk explains the finer points of the protocol and the science theories and findings behind it.
Download the Born Free Protocol PDF
Join the Born Free Community on Discord
Watch Joshua Leisk's Born Free Protocol Presentation (August 2023)
Sanitas Est Libertas.
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